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Fig. 6. 14-3-3{epsilon} had effects similar to those of 14-3-3ß on the subcellular localization of CDC25B. HE293 cells were transfected with (A) wild-type CDC25B, (B) the 309S mutant or (C) the S309A mutant with or without 14-3-3{epsilon}. Over 200 cells expressing CDC25B were counted to determine the percentage of cells that express CDC25B with nuclear, diffuse and cytoplasmic distribution. Error bars in graphs represent the means±s.d. of three independent experiments. Subcellular distribution of CDC25B: N>C, predominantly nuclear; N=C diffuse; N<C, predominantly cytoplasmic (C).





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