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Fig. 1. PHB2 specifically interacts with Akt. (A) Full-length PHB2 and the truncated PHB2 found in the yeast two-hybrid screen. The numbers represent the positions of amino acids. Abbreviation: TM, potential transmembrane domain. (B) Cos-7 cells were co-transfected with xp-PHB2 together with either HA-Akt or HA-MKK6. (C) Cell lysates containing xp-PHB2 were mixed with C2C12 WCEs harvested before and after differentiation. Immunoprecipitation and immunoblot were carried out as indicated. Abbreviations: CoIP, co-immunoprecipitation; GM, growth medium; IP, immunoprecipitation; IB, immunoblot; SF, serum-free medium; Xp, Xpress tag. Input represents 10% of total lysates used for immunoprecipitation.





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