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Fig. 4. PHB2 interacts with both MyoD and MEF2 in cells. (A) Cos-7 cells were co-transfected with various expression vectors as indicated. (B) Equal amounts of Cos-7 cell lysates expressing xp-PHB2 were separately incubated with C2C12 cell lysates harvested before and after differentiation. Xp-PHB2 was immunoprecipitated and the co-precipitated MyoD and MEF2 were detected by immunoblot. Abbreviations: GM, growth medium; SF, serum-free medium. Input represents 10% of total lysates used for immunoprecipitation. (C) Yeast AH109 cells were transformed with expression vectors as indicated. The plus and minus signs denote growth and no growth, respectively, on SD/-his/-ade/-trp/-leu plates. AD, pGADT7 vector encoding the Gal4 transcription-activation domain; BD, pGBKT7 vector encoding the Gal4 DNA binding domain.
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