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Fig. 6. PHB2 specifically interacts with HDAC1 in cells. (A) Cos-7 cells were co-transfected with various expression vectors as indicated. Either PHB2 or JNKK2 (control) were first immunoprecipitated with the anti-Xpress antibody and the co-precipitated HDAC1 was detected by immunoblot with the anti-Flag antibody. Input represents 10% of total lysates used in the immunoprecipitation. (B) C2C12 cells were co-transfected with PHB2-GFP and Flag-HDAC1. PHB2 was visualized by autofluorescence (green) of GFP and HDAC1 was visualized by indirect immunofluorescence (red) with the anti-Flag antibody. Arrows indicated cells containing both PHB2 and HDAC1. Abbreviations: GFP, green fluorescent protein. Notice the two PHB2-positive cells at the bottom left corner without HDAC1 showing predominant mitochondrial staining.





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