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Fig. 1. Stable expression of P35 by transfected RAW 264.7 mouse macrophage cells as detected by RT-PCR of total RNA (A). Lane 1, clone pCMV-FLAG (vector control); lane 2, clone pCMV-FLAG/p35#3; lane 3, pCMV-FLAG/p35#7; lane 4, clone pCMV-FLAG/p35#9. (B) Immunoblot analysis of FLAG-P35 expression. Extracts (50 µg) from stably transfected vector or P35 RAW 264.7 cells were immunoblotted with anti-FLAG M2 antibodies to detect the expression of P35. Lane 1, clone pCMV-FLAG (vector); lane 2, clone pCMV-FLAG/p35#3; lane 3, pCMV-FLAG/p35#7; lane 4, clone pCMV-FLAG/p35#9.





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