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Fig. 4. P35 expression in RAW 264.7 cells inhibits apoptosis and loss of cell viability. RAW 264.7 cells or vector- or p35-transfected clones (1x10⁶ cells/well) in 6-well tissue culture plates were treated with cisplatin (2 µg/ml) and IFN- (25 U/ml), or LPS (1 µg/ml) and IFN- (25 U/ml) in the presence or absence of L-NMMA (1 mM) as before. Cells were also treated directly with NO donor, SNP (1 mM). After 36 hours of treatment, cells were assayed for apoptosis (A) and relative cell viability (B). Data shown are mean±s.e.m. and are representative of three independent experiments done in triplicate. ^*P<0.05 versus values of control cultures. ^**P<0.05 versus values of cisplatin and IFN-/LPS and IFN--treated cultures. ^#P<0.05 versus values for cisplatin and IFN-/LPS and IFN- or SNP-treated RAW 264.7/pCMV-FLAG cells.

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