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Fig. 1. Deletion constructs of SHOX cDNA and their subcellular localization. (A) Schematic representation of deletion constructs generated in pcDNA4/HisMax and their subcellular localization. Nu, nuclear; Cy, cytoplasmic. (B) Fluorescent images of immunostained slides. The first and third rows of images show the cell nuclei stained with Hoechst dye (blue) and the second and fourth rows of images show green staining for the SHOX deletion constructs immunostained with anti-His (green) antibodies. Note that all the constructs containing the homeodomain are localized in the nucleus. Arrows indicate subcellular localization of the SHOX deletion constructs (green) and the nuclei (blue) in corresponding cells.