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Fig. 3. Analysis of the wild-type SHOX and deletion construct SHOX-NLS and the respective subcellular localization. (A) Schematic representation of the wild-type SHOX and the deletion mutant SHOX-NLS where the NLS has been deleted from the wild-type SHOX. Nu, nuclear; Cy, cytoplasmic. (B) Fluorescent images of immunostained cells. The two upper panels show the cell nuclei stained with Hoechst dye (blue) and the two lower panel shows SHOX immunostaining with rabbit anti-SHOX antibodies (green). The constructs in pcDNA4/TO were overexpressed in U2Os cells. By contrast to the wild-type SHOX, which was found in the nucleus, deletion of the nuclear localization signal from the wild-type SHOX resulted in its cytoplasmic localization. Arrows indicate subcellular localization of the SHOX constructs (green) and the nuclei (blue) in corresponding cells.





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