spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 5. Western blot analysis using cytoplasmic and nuclear extracts from U2Os cells transiently transfected with wild-type and mutant SHOX constructs. Immunofluorescence results using wild-type and mutant SHOX constructs were confirmed and complemented by western blots using cytoplasmic (Cy) and nuclear (Nu) extracts. Pyruvate kinase isozyme M2 (M2PK) at 57 kDa was used as a cytoplasmic marker and C23/nucleoloin at 116 kDa was used as a nuclear marker to detect the presence of SHOX (33 kDa) in both cytoplasmic or nuclear extracts.





Right arrow Return to article