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Fig. 1 Site-directed mutagenesis. The figure compares nucleotide sequences from the wild-type topo II{alpha} gene and the mutated sequences used in this study. Hydrophobic amino acid residues thought to be necessary for nuclear export were mutated to alanine using site-directed mutagenesis (gray boxes). Putative NES amino acid sequences are in boxes below their corresponding nucleotide sequences.





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