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Fig. 7. Immunoblotting of lipid binding and/or sensor proteins in HepG2 cells overexpressing SR-BI. Total proteins were extracted by 1% Triton X-100 and were loaded on 10% reducing SDS-PAGE. 150 µg of protein were loaded for the caveolin-1 immunoblot (A), while 50 µg were loaded for L-FABP (B), cytosolic NCEH (C) and SREBP-2 (D) immunoblots. Shown here are representative images of at least four independent protein extractions.





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