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Fig. 2. Overexpression of EGFP-rab11S25N inhibits ß2AR recycling from transferrin-positive endosomes. Cells transiently expressing vector (panels a,a'), EGFP-rab11S25N (panels b,b'), or EGFP-rab11WT (panels c,c') were treated with ISO for 20 minutes in serum free media, washed, and placed in serum-free media containing propranolol (5 µM) at 37°C to allow ß2AR recycling. (A) ß2ARs were allowed to recycle for 30 minutes in the presence of Alexa 594-transferrin (50 µg/ml). (B) Alexa 594-transferrin (100 µg/ml) was added to the media for 20 minutes during the incubation with ISO, washed, and both ß2ARs and transferrin were allowed to recycle for 15 minutes at 37°C. In both A and B, ß2ARs appear green and Alexa 594-transferrin appears red. Areas of colocalization of ß2ARs and transferrin appear yellow. Scale bar, 10 µm.





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