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Fig. 8. Overexpression of EGFP-rab11WT or S25N mutant does not affect sorting of LDL. (A) Cells transiently expressing vector alone (a,a') or EGFP-rab11WT (b,b') were fed DiI-LDL (20 µg/ml) for 5 minutes, washed, and chased for 45 minutes in the presence of Cy5-transferrin (25 µg/ml). Panels a and b show the localization of transferrin (green) and DiI-LDL (red). (B) 12ß6 cells transiently expressing EGFP-rab11WT were fed DiI-LDL for 5 minutes, chased for 45 minutes, then immediately fixed and labeled for LAMP-2. Panel a shows the localization of DiI-LDL (red) and LAMP-2 (green), with areas of colocalization appearing yellow. Scale bar, 10 µm.





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