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Fig. 5. Concentration-dependent localization of Syt V and Syt IX after their overexpression in INS-1E cells. INS-1E cells were transiently transfected with 1 µg and 5 µg of cDNAs encoding myc-tagged Syt V (A) or myc-tagged Syt IX (B). Two days later, the cells were double stained with antibodies directed against insulin (revealed by FITC-conjugated antibody) and against the myc-epitope (detected by rhodamine-conjugated antibody). Images were obtained by confocal microscopy. Yellow images correspond to the overlay of the anti-insulin and anti-myc signals.





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