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Fig. 9. Effect of Syt V and Syt IX silencing on hormone secretion in INS-1E cells. INS-1E cells were transiently cotransfected with a plasmid encoding hGH and with the empty vector (control) or with either the Syt V-specific siRNA (RNAi Syt V) or the Syt IX-specific siRNA (RNAi Syt IX). Three days later, the cells were incubated for 30 minutes with 2.5 mM glucose (open bars) or stimulated with 15 mM glucose (filled bars). The experiments depicted in the lower panel were performed in the presence of 100 nM PMA. The amount of hGH released during the incubation period was determined by ELISA. The results correspond to the mean+s.e.m. of three independent experiments performed in triplicate.
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