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Fig. 4. To determine if siRNA functioned in HMEC-1 cells, a CMV-GFP gene and its corresponding siRNA were simultaneously transfected into a monolayer of HMEC-1 cells, followed by incubation for 24 hours. A subset of these HDME-1 cells (A,B) were then placed onto matrigel and analyzed for tube formation 12-24 hours later (C,D). GFP siRNA successfully knocked down translation of GFP. Note: GFP siRNA did not affect tube formation. (E-H) As a control, GFP was expressed in HMEC-1 cells without the addition of GFP siRNA. In contrast to cells transfected with CMV-GFP and siRNA, cells without GFP siRNA were found expressing GFP both in monolayers (F) and in their corresponding tubes (H). Bar in A, for A-D, 20 µm; bar in E, for E-H, 20 µm.
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