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Fig. 5. Microvascular tube formation was compared in the absence and presence of DP siRNA. (A,E) HDME-1 cells in a monolayer do not form tubes. The absence (A) or presence (E) of DP siRNA did not affect the monolayer. (B-D) Cells placed onto a thin layer of matrigel form tubes after 5-8 hours, which persist up to 10 days in culture. Addition of 250 ng of DP siRNA significantly compromised tube formation (E1-4; F-H). E1-4 are four frames taken from a five hour video showing migration patterns of DP siRNA treated cells after plating onto matrigel. Four to five cells within the white bracket in E-1 are joined by a migrating cell (arrowhead E1-4) but do form a tube. The arrow in E1-4 points to another cell migrating towards a stationary cell. Adhesion between cells appears to be compromised up to 72 hours post siRNA treatment (H). Bars, 50 µm (A,B,E,F); 30 µm (C,G); 15 µm (D,H).
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