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Fig. 6. Western blot shows the affect of DP siRNA. (A) DP (~250/230 kDa, arrows) is absent in siRNA-treated microvascular tubes. (B) Antibodies to tubulin were used as a loading control. Tubulin runs at ~55 kDa (arrowhead). Lane 2: monolayer no DPsiRNA. Lane 3: microvascular tubes 24 hours after plating onto matrigel (48 hours after DP siRNA treatment). Lane 4: microvascular tubes 72 hours after plating onto matrigel. Lane 5: microvascular tubes 96 hours after plating onto matrigel. Lane 6: 108 hours after plating. Lane 7: 120 hours after plating. Lane 8: 144 hours after plating. Lane 9: whole mouse skin. (C-E) Western blot showing VE-cadherin staining (C), plakoglobin (D), and vimentin (E) after incubation in DPsiRNA for specified times. C-E are from the same western blot.





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