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Fig. 1. Effect of EtBr on growth of Dictyostelium discoideum Ax-2 cells. Various concentrations of EtBr were added to exponentially growing cells (2x105 cells/ml) in axenic growth medium (PS medium), followed by cell counts under a haemocytometer. () control (non-EtBr-treated cells); ({circ}) cells treated with 10 µg/ml of EtBr, ({blacktriangleup}) 20 µg/ml of EtBr, ({triangleup}) 30 µg/ml of EtBr, ({blacksquare}) 40 µg/ml of EtBr and ({square}) 50 µg/ml of EtBr. Similar results were obtained by cell counts in three independent experiments.





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