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Fig. 9. Impaired prespore differentiation in slugs derived from 
cells. Non-treated Ax-2 cells and cells were separately washed twice in BSS and incubated for 16 or 28 hours on 1.5% agar to obtain respective slugs. This was followed by immunostaining of dissociated slug cells with FITC-conjugated anti-D. mucoroides spore IgG, as described in Materials and Methods. (A) Slug cells derived from nontreated Ax-2 cells. Prespore cells have many strongly stained granules (PSVs) in the cytoplasm. (B) In slug cells derived from cells, however, it is clear that the number-ratio of prespore cells with PSVs is considerably decreased. In addition, both the number of PSVs in each prespore cell and the strength of fluorescent FITC in each PSV are lower (arrows) compared with those in slug cells derived from nontreated Ax-2 cells. Bars, 20 µm.
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