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Fig. 5. Percentage of adherent cells increases with decreasing pitch size. (A) SV40-HCEC adhesion using DPBS solution as circulating medium and a stepped-up fluid shear stress profile. Percentage of cell attachment on the seven topographical areas at all the wall shear stresses sampled. Mean±s.e.m. n=4. (ii) Percentage of cell attachment on the seven topographical areas at 40 and 80 Pa wall shear stress. Mean±s.e.m. n=4. Total cell count for each topographical area was approximately 160. Asterisks indicates significantly different means (P≤0.05). (B) SV40-HCEC adhesion using SHEM media solution as the circulating medium and a constant fluid shear stress of 10 Pa. Representative populations of cells before (i) and after (ii) fluid shear. (C) Comparison between primary and SV40 human corneal epithelial cell attachment on the 400 nm pitch after fluid shear as shown in Fig. 2B.





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