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Fig. 4. Staining for the intermediate filament proteins, desmin and vimentin, is also perturbed in MURF-2 antisense-treated cardiac myocytes. (A) Chick cardiac myocytes treated with control (a,b,d,e,g,h) or MURF-2 antisense oligonucleotides (c,f) were co-stained for desmin (b,c,g), vimentin (e,f,h) and Glu-tubulin (a,d). Desmin and vimentin staining was detected as filaments (b,e; double arrows) that colocalized with a portion of Glu-tubulin staining (a,d; merged insets in b, e; red is Glu-tubulin staining and green is desmin or vimentin), as well as striated at the Z-line regions (g,h) and at cell junctions (data not shown). In MURF-2 antisense treated myocytes, staining for desmin and vimentin was dim and diffuse (c,f). (B) Human GFP-MURF-2 expression rescues desmin staining in antisense-treated chick myocytes. Chick cardiac myocytes co-transfected with MURF-2 antisense oligos and human GFP-MURF-2 also were stained for desmin. Myocytes expressing GFP-hMURF-2 (i) contained bright filamentous desmin staining (j), compared to antisense-treated myocytes not expressing GFP-h-MURF-2 (Fig. 4A,c). Bar, 10 µm.
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