QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 6. MURF-2 colocalizes with a portion of microtubules and with assembling myofibrils in primary cultures of skeletal myocytes. Myofibrillogenesis in skeletal myogenic cells was analyzed by costaining myotubes for ${alpha}$ -actinin (d,f and data not shown). MURF-2 staining was mainly diffuse in the cytoplasm throughout the culture period (a,c,e). In addition, some MURF-2 staining was detected colocalized with ${alpha}$ -tubulin staining early in culture (a,b; merged inset in b; red is MURF-2 staining and green is tubulin; double arrows point to colocalization). Later during myofibrillogenesis, MURF-2 staining was detected at the M-line region, although in relatively few myofibrils (c,d; merged image in d; MURF-2 staining in red, ${alpha}$ -actinin staining is green; double arrows point to myofibrils). In mature myotubes, MURF-2 staining also could be detected along the length of some myofibrils (e,f; double arrowheads). Bar, 10 µm.

Return to article