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Fig. 5. Quantitative analysis of syndecan-4 endocytosis was carried out using confocal microscopy as described in the Materials and Methods. The results are presented as a fold change from baseline for each substance. (A) The effects of dominant negative constructs (AP180c) and dynamins 1 and 2 (Dyn1^K44A and Dyn2^K44A) on clathrin-dependent uptake of syndecan-4 (black bars) and transferrin (gray bars). Note inhibition of transferrin but not syndecan-4 uptake. * P<0.05 versus baseline. (B) The effects of Rac1 and Cdc42 dominant negative and constitutively active constructs and amiloride treatment on FcR-syndecan-4 (black bars), FGF2 (gray bars) and dextran (white bars) uptake was examined in RFPEC. Note a significant inhibition of syndecan-4 and FGF2 uptake in Rac1-DN-expressing cells and increased uptake in Rac1-CA expressing cells. Cdc42-DN and amiloride inhibited uptake of all three substances. *P<0.05 versus baseline endocytosis.

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