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Fig. 9. Syndecan-4 and FGF2 uptake proceed via macropinocytosis. FcR-S4-expressing RFPEC cultured with fluorescent dextran (red, a) were decorated by niIgG (green, b) followed by FGF2 (blue, c) clustering. Merged images demonstrate colocalization of dextran and syndecan-4 (d), dextran and FGF2 (e) and all three signals (f). Amiloride pretreatment completely inhibited endocytosis of both dextran (g) and FcR-S4 (h-j). (k) The merged image overlaid with the DIC image. Scale bars, 10 µm.





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