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Fig. 5. Pre-treatment of RTG-2 and CHSE-214 cells with the antioxidant N-propylgallate (nPG; 20 µM) for 30 minutes increases the amount of HIF-1{alpha} protein after CoCl2 (200 µM) treatment. Cells were first treated in the presence or absence of nPG and then with CoCl2 (2 hours) under normal oxygen conditions (21% O2). Cobalt treatment in the absence of nPG caused an increase in the level of HIF-1{alpha} protein. The level of HIF-1{alpha} protein was further increased in the presence of nPG. Furthermore, the HIF-1{alpha} protein band was broadened in the presence of nPG, a finding compatible with redox-sensitive phosphorylation of the protein. The HIF-1{alpha} protein was detected using immunoprecipitation techniques with trout-specific HIF-1{alpha} antibody.





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