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Fig. 10. Association of cadherin with ß-catenin and PTP1B in mouse tissue and effect of EGF on D743R cells. (A) Brain lysates from wild-type or ferD743R mice were immunoprecipitated with anti-pan-cadherin antibody and analysed by western blot with the indicated antibodies. The relative intensities of the cadherin, ß-catenin and PTP1B bands were determined by densitometry and are presented as ratios. (B) WT or D743R cells grown to semiconfluence were washed free of serum and incubated with or without 100 ng ml–1 EGF for 2 hours. The cells were then lysed in neutral detergent, immunoprecipitated with anti-pan-cadherin antibody and analysed by western blot with anti-ß-catenin and anti-PTP1B antibodies. (C) Alternatively, cells were lysed in RIPA buffer, immunoprecipitated with anti-ß-catenin antibody and analysed by western blot with anti-phosphotyrosine antibody.
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