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Fig. 2. Binding of p120ctn to Fer. (A) Full-length and GST constructs of p120ctn used in the in vitro binding assay. Filled rectangles represent the Armadillo domains. (B) Recombinant GST-p120ctn peptides conjugated to biotin were immobilized on streptavidin-coated wells and incubated with His-tagged Fer-3. Bound protein was determined by ELISA using anti-His-tag antibody and HRP-conjugated second antibody. (C) Dose response of binding of the biotinylated GST-p120ctn peptides to His-Fer-3. His-Fer-3 was incubated with increasing concentrations of immobilized GST-p120ctn constructs 1 to 10, and bound protein was determined as described in B.





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