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Fig. 4. Effect of cell permeable Antennapedia fusion peptides that mimic the Fer-p120ctn binding site on cell adhesion and neurite outgrowth. (A,B) Adhesion of E8 chick retina cells to cadherin (A) and laminin (B). Dissociated E8 chick neural retina cells were incubated for 1 hour at room temperature in the presence of the indicated peptide. Equal numbers of cells were then aliquoted into wells precoated with the extracellular domain of N-cadherin, Fc-N-cad (A) or with laminin (B) and incubated for 1 hour in the presence of the indicated peptides. Adherent cells were stained with crystal violet and quantified by absorbance at 560 nm. No adhesion is observed onto uncoated, blocked wells (No Ncad). Adhesion is expressed as the percentage of adhesion in the absence of added peptides (A) or in the presence of control peptide FerCo (B). (C,D) Neurite outgrowth. E7 chick retina cells were plated on slides coated with anti-N-cadherin antibody NCD-2 or laminin. Single cells bearing neurites longer than two cell diameters were counted and expressed as a percentage of the total adherent cells (C). Cells and neurites were visualized under phase contrast (D).
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