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Fig. 7. FTI treatment has no obvious short-term effect on signalling through the EGF receptor. Cells were serum starved overnight in the absence or presence of FTI and then stimulated with 100 ng/ml EGF for the indicated times. Activation of Erk1/2, PKB, JNK and p38 was analyzed by western blot using phospho-specific antibodies. The blots are representative of at least three separate experiments.