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Fig. 10. Failure of hamster GFP-CHO1-4A to rescue the CHO1-depletion-induced cytokinesis defect. (A,B) HeLa cells were first transfected with pBS/U6-CHO1 to deplete the endogenous CHO1. After overnight incubation, cells were transfected with either hamster GFP-CHO1-WT (A) or GFP-CHO1-4A (B) for 24 hours. Cells were fixed and subjected to anti-Plk1 staining. (C) The protocol used to rescue the CHO1-depletion-induced cytokinesis defect by hamster GFP-CHO1 in well-synchronized cells. (D,E) Histograms quantifying the results. Scale bar, 10 µm.





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