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Fig. 6. Cytokinesis defect in CHO1-depleted cells. (A,B) HeLa cells were transfected with control vector (A) or pBS/U6-GFP-CHO1 (B), in which GFP was independently expressed as a marker for transfected cells. Only the GFP-positive cells produced hairpin RNA to induce CHO1 depletion. 30 hours after transfection, cells were fixed and subjected to anti-{alpha}-tubulin staining. Arrows indicated the regions of mid-zone/mid-body structure. Scale bar, 10 µm. (C) The protocol used to deplete CHO1 in well-synchronized cells. (D) Histogram quantifying the results.





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