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Fig. 7. Multinucleation induced by overexpression of unphosphorylatable CHO1 mutant. HeLa cells were transfected with GFP-CHO1-WT or -4A constructs using PolyFect reagents. 48 hours after transfection, cells were fixed and subjected to anti-CHO1 or anti-Plk1 staining as indicated. (A) GFP-CHO1 expression patterns during interphase. (B) Nuclear localization of GFP-CHO1 constructs with high resolution. (C,D) Intercellular bridge localization of GFP-CHO1-WT, but not the 4A mutant. (E) GFP-CHO1 expression patterns during mitosis. (F) Plk1 localization in GFP-CHO1-overexpressing cells. (G) Representative cells with overexpressed GFP-CHO1-4A. (H) Histogram quantifying the results. Scale bar, 10 µm.





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