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Fig. 8. Dominant negative effect of Plk1-unphosphorylatable mutant CHO1 on cytokinesis. (A) The protocol used to overexpress CHO1 constructs in well-synchronized cells. (B) Representative images to show cells with overexpressed GFP-CHO1 using the protocol in (A). Arrows indicate the positions of mid-bodies. (C) Representative image to show cells with overexpressed GFP-CHO1-4A using the protocol in (A). (D) Histogram quantifying the results. Scale bar, 10 µm.





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