spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 9. Effect of Plk1-unphosphorylatable mutations on the rescue ability of hamster CHO1/MKLP-1 toward CHO1-depletion induced multinucleation in HeLa cells. (A) Sequence alignment of RNAi targeting site between human and hamster MKLP-1. The nonconserved sites are indicated in bold. (B) HeLa cells were cotransfected with hamster vectors that express CHO1 as indicated on the left. Three days after transfection, cells were harvested and subjected to FACS analysis. GFP-positive cells were gated and the proportion of cells with 8N DNA content was calculated. (C) Histogram showing results from six independent experiments; bars indicate standard deviations. (D) Representative cells with overexpressed GFP-CHO1 constructs in the absence of endogenous CHO1. (E) Histogram quantifying the results.





Right arrow Return to article