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Fig. 5. Acute regulation of testicular resistin mRNA expression by gonadotropins. (A) A representative RT-PCR assay of the testicular expression levels of resistin mRNA at different time-points (2-24 h) after exposure to 25 IU hCG in vivo. (B) A representative RT-PCR assay is shown of testicular resistin mRNA levels at different times (2-24 hours) after exposure to 12.5 IU recombinant FSH in vivo. Semi-quantitative values are the mean±s.e.m. of at least four independent determinations. a,b and c denote groups that ar e statistically different (P<0.01; ANOVA followed by Tukey's test).