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Fig. 6. Transverse cryosections from muscles transplanted with population C MPCs. TA muscles were irradiated and injected with retrovirally infected isolated fibre MPCs (population C). Three weeks after cell transplantation, the right TA muscles (a,b,e,f) were treated with notexin and the muscles analysed seven days later. (a) Immunostained for dystrophin and counterstained with hematoxylin. Many small, newly regenerated dystrophin-positive fibres are present (small arrow), with a few undamaged dystrophin-negative host fibres (large arrow). The section also contains a large area of undifferentiated cells and necrotic muscle fibres (*). Magnificatiox10. (b) Serial section to (a) stained for ß-gal. (c,d) Left TA muscles [contralateral to (a) and (b)] that had been injected with MPCs but had not been treated with notexin. (c) Stained for dystrophin and (d) stained for ß-gal. Magnification x10. (e) Stained for dystrophin. Magnification x20. (f) Stained for ß-gal. Magnification x20.





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