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Fig. 11. HGEC adhesion to collagen IV or laminin in the presence of anti-PC antibody. [35S] methionine labeled HGEC grown in either 5 mM (A) or 25 mM (B) glucose, were allowed to adhere to collagen IV or laminin substrates in the presence of serial dilutions of 3D3 anti-PC antibody (1:50-1:2500). Control cells adhered in the absence of antibodies. Adherent cells were lysed and lysates were counted in a scintillation counter. Each value was plotted relative to the extent of cell adhesion achieved in the absence of antibodies, and represents the mean±s.d. of three independent experiments. Control values (adhesion in the absence of antibodies) were expressed as 100% adhesion. Single factor ANOVA and additionally SNK test were used independently in each experiment to determine whether the effect of monoclonal antibodies on cell adhesion was significant (*P<0.05).





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