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Fig. 12. Cell adhesion to collagen IV in the simultaneous presence of anti-ß1 integrin and anti-PC monoclonal antibodies. [³⁵S] methionine labeled HGEC cultured in either 5 mM (A) or 25 mM (B) glucose, were allowed to adhere to collagen IV in the presence of anti-ß1 integrin monoclonal antibody which was used at 1:400 and 1:1500 dilution. The effects of anti-ß1 antibody on HGEC adhesion were examined in the presence or absence of serial dilutions of 3D3 anti-PC monoclonal antibody. Control cells adhered in the absence of mAbs. Adherent cells were lysed and lysates were counted in a scintillation counter. Each value, was plotted relative to the extent of cell adhesion achieved in the absence of antibodies, and represents the mean±s.d. of three independent experiments. Control values were expressed as 100% adhesion. Single-factor ANOVA and additionally Newman-Keuls test were used independently in each experiment to determine whether the effect of anti-PC monoclonal antibody on inhibition of cell adhesion, caused by anti-ß1 integrin mAb, was significant (*P<0.05).

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