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Fig. 4. Confocal microscopic analysis of PC distribution in HGEC. Cells were cultured on coverslips either uncoated (control) or coated with 20 µg/cm2 of each laminin or GBM substrate. HGEC were fixed in formaldehyde and stained with 3D3 anti-PC antibody diluted 1:50 in PBS and fluorescein-conjugated anti-mouse IgG as a secondary antibody. Panels a-c correspond to serial X-Y sections of the basal surface generated with a motor step of 0.5 µm. Panels d correspond to the lateral surface, and panels e to the apical surface. Scale bar, 15 µm.





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