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Fig. 6. Confocal microscopic analysis of the distribution of ZO-1 in HGEC cultured on GBM. Cells were cultured on coverslips coated with 20 µg/cm2 of GBM substrate. HGEC were fixed in formaldehyde, permeabilized with Triton-X100 and stained with anti-ZO-1 antibody at a concentration of 5 µg/ml in PBS, 5% FCS and FITC-conjugated anti-mouse IgG as a secondary antibody. Scale bar, 10 µm.





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