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Fig. 2. Single confocal sections showing the comparison of the disassembly level of various sarcomeric proteins present in cultured cardiomyocytes during metaphase (B,D,F,H,J,L,N). While cardiac {alpha}-actin (E) as well as Z-disk associated epitopes like {alpha}-actinin (A) and titin T12 (C) display a mostly diffuse staining pattern throughout the entire cytoplasm already at this stage; sarcomeric myosin heavy chain (G), myosin binding protein-C (I) and M-band-associated epitopes, such as myomesin (K) and titin T51 (M), show an intact localization pattern. Bar represents 10 µm.





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