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Fig. 3. Localization pattern of different sarcomeric proteins in cultured cardiomyocytes during later stages of mitosis in single confocal sections. Late metaphase/early anaphase was visualized by staining for phosphorylated histone H3 (D-F), the later stages of mitosis by staining for tubulin (J-L and P-R). Staining for the sarcomeric protein cardiac 
-actin is shown in the left column, in the middle is myomesin and on the right is sarcomeric myosin heavy chain, as indicated above. While cardiac -actin (A) is completely diffuse at late metaphase, myomesin (B) and sarcomeric myosin heavy chain (C) only start to disassemble at this stage (small arrowheads point at still intact myofibrils). Only when chromosomes are being segregated, cardiac -actin (G), myomesin (H) as well as myosin heavy chain (I) show a diffuse pattern with some remaining aggregates, especially in the case of the latter. During cytokinesis, as identified by tubulin concentration in the midbody (P-R), all sarcomeric proteins start reassembly to myofibrils and display a cross-striated pattern again (M-O). The arrows point at the dividing cells. Bar represents 10 µm.
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