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Fig. 4. Sequential disassembly of different parts of the sarcomere analyzed in single confocal sections. Embryonic rat cardiomyocytes were stained for ${alpha}$ -actinin (B,E,H,K; red in overlay A,D,G,J) and for MyBP-C (C,F,I,L; green in overlay) as indicated above the columns. The stage of cell division was identified by staining for tubulin (blue in overlay). In the interphase cell, clear cross-striations can be seen for both sarcomeric proteins. At metaphase the localization pattern of ${alpha}$ -actinin is already diffuse (arrow), while double bands are still visible for MyBP-C (arrowheads), which only redistributes by the anaphase and telophase stage. Bar represents 10 µm.

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