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Fig. 7. Disassembly of myofibrils is delayed in cardiomyocytes that were treated with MG132 to inhibit proteasome degradation. Single confocal sections of cardiomyocytes stained with monoclonal antibodies to sarcomeric {alpha}-actinin (B,D; red in A,C) and for tubulin (blue in A,C) as well as for phosphorylated histone (green in A,C) to identify the stage of mitosis. While in control cells at metaphase all the {alpha}-actinin is localized in a diffuse fashion throughout the entire cell (A,B), cross-striated myofibrils can still be seen in MG132 treated cardiomyocytes (C,D). Bar represents 10 µm.





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