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Fig. 3. Expression of claudins in freshly isolated RPE, retina and choroid. (A,B) The RT-PCR reaction was used to amplify claudin mRNAs from the indicated tissues. The positions of standards derived from a Hae III digest of 
X174 (603, 281 and 118 base pairs) are indicated on the right. A single reaction product of the expected length was obtained in each case. No reaction product was detected when reverse transcriptase was omitted from the reaction (data not shown). (A) Ocular tissues. (B) Claudins undetected in ocular tissues could be amplified from other tissues. (C) Real-time RT-PCR was used to quantify the amount of claudin mRNA. Data points, with the standard error, represent the average of 6-9 experiments. (D) RPE stained for the indicated claudin were viewed en face by epifluorescence microscopy. Double staining with ZO-1 (not shown) was used to adjust the focal plane to the junctional region of the lateral membranes. Bar, 10 µm.
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