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Fig. 4. E14 retinal conditioned medium promotes the formation and assembly of tight junctional strands in RPE that is cultured in minimal medium. RPE was isolated from E7 (A,B) and E14 (C,D) embryos and cultured with SF3 medium in the basal chamber and either SF3 (A,C) or rcSF3 (B,D) in the apical chamber. (A) A loose, shallow network of strands was commonly observed in E7 cultures in SF3. (B) When rcSF3 was added to the apical medium chamber, the depth, complexity and number of strands parallel to the apical membrane increased. (C) A rare image from E14 cultures in SF3 shows a loosely organized cluster of junctional strands. (D) When rcSF3 was added to the apical medium chamber, continuous, well-organized networks of tight junctional strands were always observed. Arrowheads, discontinuity; EF, E-face; PF, P-face. Bar, 0.25 µm.





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