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Fig. 5. Quantification of the effects of rcSF3 on the morphology of tight junctions. Strand number (A,C) and depth (B,D) were determined for the E7 (A,B) and E14 (C,D) cultures depicted in Fig. 4. The apical medium chamber contained either SF3 (
) or rcSF3 (
). In E7 cultures, the junctions were heterogeneous with respect to strand number and depth. Although the junctional networks remained heterogeneous, retinal conditioned medium increased the number of strands and the depth. In E14 cultures, the rare junctions that were observed in the absence of rcSF3 had a structure that was similar to those found in the presence of rcSF3. In E14 cultures, the principal effect was to convert the rare patches into the continuous, circumferential network that typifies tight junction morphology.
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