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Fig. 8. Effect of PDZ domain proteins NHERF1/2 on the apical surface lateral mobility of non-JN NHE3-EGFP. Photobleaching experiments were done on OK/NHE3-EGFP (A), OK/NHERF2/NHE3-EGFP (B) and OK/NHERF2/NHE3585-EGFP cells (C). Initial fluorescence was set to 100%. The estimated mobile fractions and effective diffusion coefficients are shown in (D) and (E). P values are in comparison with OK/NHE3-EGFP (unpaired t-test). n=21 for OK/NHE3-EGFP, n=11 for OK/NHERF2/NHE3-EGFP and n=9 for OK/NHERF2/NHE3585-EGFP cells from at least three separate experiments. NS, not significant.





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