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Fig. 5. Expression of wild-type and category-II (CAP-associated) mutants in NIH3T3 fibroblasts. Wild-type actin (A; scale bar, 25 µm) was transfected as a control and was incorporated into stress fibers, ruffles and lamellipodia, and generally co-localized with the endogenous filamentous actin, as visualized with phalloidin (A',A''). The mutant G182D showed a dotted pattern in the cytoplasm (B) and co-localized with the endogenous actin in stress fibers and lamellipodia (B',B''). G15R was partially incorporated into the stress fibers and lamellipodia (C). In some cells, the mutant protein formed aggregates that stained with phalloidin (C,C'', arrow). (A''-C'') Red corresponds to the Myc staining and green to the phalloidin staining. (A'',C'') Nuclei were stained with Hoechst and appear blue.
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